Dr. Amit Kumar Singh
- Area Of Interest
- Award & Recognition
- Research & Publications
My area of research is structural and functional studies of target proteins in order to understand their biological functions and subsequent functional implication. I am particularly interested in purification, characterization, crystallization and structural studies of proteins through X-ray crystallography in combination with wide range of biophysical techniques to investigate the structural and functional relationship of targeted proteins.
I am also interested in the structural and function studies of mammalian heme peroxidases and their role in breast carcinogenesis and hyperthyroidism. I am also interested in characterizing and determining the structures of potential therapeutic and antimicrobial proteins from various natural sources.
- 5 years
- Protein Structure Biology and Drug Discovery
INSA Young Scientist Medal Award, 2012 from Indian National Science Academy (INSA), New DelhiDST Young Scientist Fellow Award, 2010 from Department of Science and Technology (DST), New DelhiSelected, Nominated and supported by Indian National Sciences Academy (INSA) and Israel Academy of Sciences and Humanities to attend the training workshop on “Sharing the Start-up Experience” at the Israel Academy of Sciences and Humanities in Jerusalem, Israel from 25th Nov to 28th Nov 2013. Won ‘Best Poster Award’ from NSC-36, Chennai, 2007 from National Seminar on CrystallographyQualified CSIR-NET-JRF (Junior Research Fellow), June 2004Qualified UGC-JRF (Junior Research Fellow), December, 2004Qualified CSIR-NET-LS (Lecturer Ship), June 2002Awarded the GATE examination 2003, 2004 and 2005
- Singh, A.K., Singh, N., Sharma, S., Singh, S.B., Kaur, P., Bhushan, A., Srinivasan, A. and Singh, T.P. (2008) Crystal structure of lactoperoxidase at 2.4 Å resolution: J. Mol. Biol., 376, 1060-1075.
- Singh, A.K., Singh, N., Sharma, S., Shin, K., Takase, M., Kaur, P., Srinivasan, A. and Singh, T.P. (2009) Inhibition of lactoperoxidase by its own catalytic product: crystal structure of the hypothiocyanate-inhibited bovine lactoperoxidase at 2.3 Å resolution: Biophys. J., 96, 646-654.3. Sheikh, I.A.,
- Singh, A.K., Singh, N., Sinha, M., Singh, S.B., Bhushan, A., Kaur, P., Srinivasan, A., Sharma, S. and Singh, T.P. (2009) Structural evidence of substrate specificity in mammalian peroxidases: structure of the thiocyanate complex with lactoperoxidase and its interactions at 2.4 Å resolution: J. Biol. Chem., 284, 14849-14856.
- Singh, A.K., Singh, N., Sinha, M., Bhushan, A., Kaur, P., Srinivasan, A., Sharma, S. and Singh, T.P. (2009) Binding modes of aromatic ligands to mammalian heme peroxidases with associated functional implications: crystal structures of lactoperoxidase complexes with acetylsalicylic acid, salicylhydroxamic acid, and benzylhydroxamic acid: J. Biol. Chem., 284, 20311-20318.
- Singh, A.K., Kumar, R.P., Pandey, N., Singh, N., Sinha, M., Bhushan, A., Kaur, P., Sharma, S. and Singh, T.P. (2010) Mode of binding of the tuberculosis prodrug isoniazid to peroxidases: Crystal structure of bovine lactoperoxidase with isoniazid at 2.7 Å resolution: J. Biol. Chem., 285, 1569-1576.
- Singh, A.K., Singh, N., Tiwari, A., Sinha, M., Kushwaha, G.S., Kaur, P., Srinivasan, A., Sharma, S. and Singh, T.P. (2010) First structural evidence for the mode of diffusion of aromatic ligands and ligand-induced closure of the hydrophobic channel in heme peroxidases: J. Biol. Inorg. Chem., 15, 1099-1107.
- Singh, A.K., Pandey, N., Sinha, M., Kaur, P., Sharma, S. and Singh, T. P. (2011) Structural evidence for the order of preference of inorganic substrates in mammalian heme peroxidases: crystal structure of the complex of lactoperoxidase with four inorganic substrates, SCN-, I-, Br- and Cl- : Int. J. Biochem. Mol. Biol. 2, 328-339.
- Singh A.K., Smith, M.L., Yamini S., Ohlsson, P., Sinha, M., Kaur, P., Sharma, S.,Paul., A.K., Singh, T.P. and Paul, K.G. (2012) Bovine Carbonyl Lactoperoxidase Structure at 2.0Å Resolution and Infrared Spectra as a Function of pH: Protein J. 2012 Oct;31(7):598-608
- Singh, A.K., Pandey, N., Sinha., M., Sharma, S., and Singh, T.P. (2011) Mammalian heme peroxidases and Mycobacterium tuberculosis. Understanding Tuberculosis III: Deciphering the secret life of the bacilli, INTECH Open Access Publisher, ISBN 979-953-307-699-6.
- P. Kaur, N. Pandey, A. K. Singh, M. Sinha, S. Sharma and T. P. Singh. (2011). First structural evidence for the order of preference of inorganic substrates by lctoperoxidase. Acta Cryst. (2011). A67, C768-C769.
- Sharma, S, Singh A.K., Kaushik, S., Sinha, M., Singh, R.P., Sharma, P., Sirohi, H., Kaur, P., Singh, T.P. (2013). Lactoperoxidase: Structural Insights into the Function, Ligand Binding and Inhibition. Int. J. Biochem. Mol. Biol. 4(3), 108-128
- Singh, R.P., Singh, A., Kushwaha, G.S., Singh, A.K., Kaur, P., Sharma, S., and Singh, T.P. (2014) Mode of binding of antithyroid drug propylthiouracil to mammalian heme peroxidases. Acta Crystallographica. D. (In Press)
- Indian Crystallographic Association (ICA), IndiaThe Biotech Research Society, India (BRSI)
It was a great time we spent in Sharda University. Sharda has very wonderful faculty and they are very cooperative and campus environment is very positive. Training and placement department continuousHarshdeep Batra MBA (2014-2015)
It gives me great pleasure to say with pride that I have completed my post-graduation from School of Business Studies, Sharda University. The relationship between faculties and student is very cordialDeeksha Gupta MBA - Marketing & Finance (2014-15)
Taking admission into Sharda is like paying for an exclusive world tour. The university is comprised of different people from different nationalities which makes it a global community for diverse learIkechukwu M. Torti MBA – International Business (2014-15)
Building my career in Sharda University enriched my life. I can somehow see that my future is totally reliant on the career for which I am grateful to every faculty member of Chemistry Department, SchKanjur Wangdi Master of Science (Integrated), Chemistry
Studying architecture in Sharda has widened my perspective and has given me an idea of the importance of architecture in today’s day and age. The faculties and staff are very helpful and are alwShreeyukti Shah Architecture, 2014-15
Architecture is a field with uniqueness and individuality, the passion for which comes within the individual. Being a part of this course in Sharda University is like know your own individuality. TheZainab Manzoor Architecture, 2014-15
Hi.. ! I am Mohd. Faizan studying in school of architecture and planning is currently in 3rd year in sharda university. It has been a great experience. Here in sharda we have great faculty membeMohd. Faizan Architecture, 2014-15
Sharda University is the best university across the india and is known for its excellence . It has all the best facilities under one roof - state of infrastructure , erudite faculty , professional teaYamini Sharma Architecture, 2014-15
I can positively say Sharda University has made me a better person. It has helped me develop a positive attitude towards my studies and discover more about myself. Teachers are very caring and inMohammad Arish Architecture, 2014-15
Hello! I I am student of Sharda University pursuing Architecture. I am currently in 3rd year. It has been a great experience overall here in Sharda. we have a panel of great faculty members who aAparna Chatterjee Architecture, 2014-15