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Dr. Amit Kumar Singh

Assistant Professor, Biotechnology

amitk.singh@sharda.ac.in

About

My area of research is structural and functional studies of target proteins in order to understand their biological functions and subsequent functional implication. I am particularly interested in purification, characterization, crystallization and structural studies of proteins through X-ray crystallography in combination with wide range of biophysical techniques to investigate the structural and functional relationship of targeted proteins.

I am also interested in the structural and function studies of mammalian heme peroxidases and their role in breast carcinogenesis and hyperthyroidism. I am also interested in characterizing and determining the structures of potential therapeutic and antimicrobial proteins from various natural sources.

Experience
  • 5 years
Qualification
  • Ph.D.
Award & Recognition

INSA Young Scientist Medal Award, 2012 from Indian National Science Academy (INSA), New DelhiDST Young Scientist Fellow Award, 2010 from Department of Science and Technology (DST), New DelhiSelected, Nominated and supported by Indian National Sciences Academy (INSA) and Israel Academy of Sciences and Humanities to attend the training workshop on “Sharing the Start-up Experience” at the Israel Academy of Sciences and Humanities in Jerusalem, Israel from 25th Nov to 28th Nov 2013. Won ‘Best Poster Award from NSC-36, Chennai, 2007 from National Seminar on CrystallographyQualified CSIR-NET-JRF (Junior Research Fellow), June 2004Qualified UGC-JRF (Junior Research Fellow), December, 2004Qualified CSIR-NET-LS (Lecturer Ship), June 2002Awarded the GATE examination 2003, 2004 and 2005

Research

  • Singh, A.K., Singh, N., Sharma, S., Singh, S.B., Kaur, P., Bhushan, A., Srinivasan, A. and Singh, T.P. (2008) Crystal structure of lactoperoxidase at 2.4 Å resolution     J. Mol. Biol., 376, 1060-1075.
  • Singh, A.K., Singh, N., Sharma, S., Shin, K., Takase, M., Kaur, P., Srinivasan, A. and Singh, T.P. (2009) Inhibition of lactoperoxidase by its own catalytic product crystal structure of the hypothiocyanate-inhibited bovine lactoperoxidase at 2.3 Å resolution Biophys. J., 96, 646-654.3.    Sheikh, I.A.,
  • Singh, A.K., Singh, N., Sinha, M., Singh, S.B., Bhushan, A., Kaur, P., Srinivasan, A., Sharma, S. and Singh, T.P. (2009) Structural evidence of substrate specificity in mammalian peroxidases structure of the thiocyanate complex with lactoperoxidase and its interactions at 2.4 Å resolution J. Biol. Chem., 284, 14849-14856.
  • Singh, A.K., Singh, N., Sinha, M., Bhushan, A., Kaur, P., Srinivasan, A., Sharma, S. and Singh, T.P. (2009) Binding modes of aromatic ligands to mammalian heme peroxidases with associated functional implications crystal structures of lactoperoxidase complexes with acetylsalicylic acid, salicylhydroxamic acid, and benzylhydroxamic acid    J. Biol. Chem., 284, 20311-20318.
  • Singh, A.K., Kumar, R.P., Pandey, N., Singh, N., Sinha, M., Bhushan, A., Kaur, P., Sharma, S. and Singh, T.P. (2010) Mode of binding of the tuberculosis prodrug isoniazid to peroxidases Crystal structure of bovine lactoperoxidase with isoniazid at 2.7 Å resolution   J. Biol. Chem., 285, 1569-1576.
  • Singh, A.K., Singh, N., Tiwari, A., Sinha, M., Kushwaha, G.S., Kaur, P., Srinivasan, A., Sharma, S. and Singh, T.P. (2010) First structural evidence for the mode of diffusion of aromatic ligands and ligand-induced closure of the hydrophobic channel in heme peroxidases J. Biol. Inorg. Chem., 15, 1099-1107.
  • Singh, A.K., Pandey, N., Sinha, M., Kaur, P., Sharma, S. and Singh, T. P. (2011) Structural evidence for the order of preference of inorganic substrates in mammalian heme peroxidases crystal structure of the complex of lactoperoxidase with four inorganic substrates, SCN-, I-, Br- and Cl-  Int. J. Biochem. Mol. Biol. 2, 328-339.
  • Singh A.K., Smith, M.L., Yamini S., Ohlsson, P., Sinha, M., Kaur, P., Sharma, S.,Paul., A.K., Singh, T.P. and Paul, K.G. (2012) Bovine Carbonyl Lactoperoxidase Structure at 2.0Å Resolution and Infrared Spectra as a Function of pH Protein J. 2012 Oct;31(7)598-608
  • Singh, A.K., Pandey, N., Sinha., M., Sharma, S., and Singh, T.P. (2011) Mammalian heme peroxidases and Mycobacterium tuberculosis. Understanding Tuberculosis III Deciphering the secret life of the bacilli, INTECH Open Access Publisher, ISBN 979-953-307-699-6.
  • P. Kaur, N. Pandey, A. K. Singh, M. Sinha, S. Sharma and T. P. Singh. (2011). First structural evidence for the order of preference of inorganic substrates by lctoperoxidase. Acta Cryst. (2011). A67, C768-C769.
  • Sharma, S, Singh A.K., Kaushik, S., Sinha, M., Singh, R.P., Sharma, P., Sirohi, H., Kaur, P., Singh, T.P. (2013). Lactoperoxidase Structural Insights into the Function, Ligand Binding and Inhibition. Int. J. Biochem. Mol. Biol. 4(3), 108-128
  • Singh, R.P., Singh, A., Kushwaha, G.S., Singh, A.K., Kaur, P.,  Sharma, S., and Singh, T.P. (2014) Mode of binding of antithyroid drug propylthiouracil to   mammalian heme peroxidases. Acta Crystallographica. D. (In Press)

Certifications

NA

Area of Intrest

  • Protein Structure Biology and Drug Discovery